In order to assess whether the use of cell lines could expedite the production process of recombinant vaccines and simplify the study of their impact on the transcriptional immune response, a group of researchers from the “Aquaculture Genomics” line at the Interdisciplinary Center for Aquaculture Research (INCAR) analyzed the transcriptomic response of SHK-1 cells and vaccinated fish with the potential vaccine candidate, cathepsin.<\/p>\n
According to the paper “Comparative Transcriptomics in Atlantic Salmon Head Kidney and SHK-1 Cell Line Exposed to the Sea Louse Cr-Cathepsin,” authored by Dr. (c) Yeny Leal, Dr. Valentina Valenzuela-Mu\u00f1oz, Dr. Antonio Casuso, B\u00e1rbara P. Benavente, and the Deputy Director and Principal Investigator of the center, Dr. Cristian Gallardo-Esc\u00e1rate, the findings revealed differences between the transcriptomic profiles of SHK-1 cells and kidney samples, but with a 24.15% identity among differentially expressed genes. However, the gene expression of long non-coding RNAs (lncRNAs) differed between the evaluated groups.<\/p>\n
Based on these results, the researchers concluded that it is possible to use this in vitro approach to evaluate candidate antigens for the development of vaccines against sea lice.<\/p>\n