In order to assess whether the use of cell lines could expedite the production process of recombinant vaccines and simplify the study of their impact on the transcriptional immune response, a group of researchers from the “Aquaculture Genomics” line at the Interdisciplinary Center for Aquaculture Research (INCAR) analyzed the transcriptomic response of SHK-1 cells and vaccinated fish with the potential vaccine candidate, cathepsin.
According to the paper “Comparative Transcriptomics in Atlantic Salmon Head Kidney and SHK-1 Cell Line Exposed to the Sea Louse Cr-Cathepsin,” authored by Dr. (c) Yeny Leal, Dr. Valentina Valenzuela-Muñoz, Dr. Antonio Casuso, Bárbara P. Benavente, and the Deputy Director and Principal Investigator of the center, Dr. Cristian Gallardo-Escárate, the findings revealed differences between the transcriptomic profiles of SHK-1 cells and kidney samples, but with a 24.15% identity among differentially expressed genes. However, the gene expression of long non-coding RNAs (lncRNAs) differed between the evaluated groups.
Based on these results, the researchers concluded that it is possible to use this in vitro approach to evaluate candidate antigens for the development of vaccines against sea lice.
For more information, please read the paper here
